CILIARY MEMBRANE DIFFERENTIATIONS IN TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
Ciliary Membrane Differentiations in Tetrahymena Pyriformis
We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections. Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associat...
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We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis . The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections . Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associ...
متن کاملStudies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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Tetrahymena ciliary membranes were prepared by four different techniques, and their protein composition was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), electron microscopy, and two-dimensional thin-layer peptide mapping. Extraction of the isolated cilia by nonionic detergent solubilized the ciliary membranes but left the axonemal microtubules and dyneine ar...
متن کاملThe Isolation of Ciliary Basal Bodies (kinetosomes) from Tetrahymena Pyriformis
S e a m a n (7, 8) has devised a m e t h o d for the isolation of ciliary basal bodies (kinetosomes) from Tetrahymena pyriformis a n d has presented d a t a on the c h e m i c a l properties of his fraction. However, only light m i c r o s c o p y was e m p l o y e d as a m e a n s of identification of the kinetosomes. I n the experi-m e n t s described here the m a t e r i a l o b t a i n e d ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.62.2.473